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KMID : 1059519910350040397
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Journal of the Korean Chemical Society
1991 Volume.35 No. 4 p.397 ~ p.404
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Separation and Detection of Nonchromophore Aliphatic Compounds by Reversed-Phase Liquid Chromatography using Ultraviolet-Absorbing Reagent
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Lee Seung-Seok
Kang Sam-Woo
Oh Hae-Beom
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Abstract
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Nonchromophore compounds such as aliphatic acids, alcohols and tetraalkylammonium salts could be detected by indirect photometric detection on the revered-phase liquid chromatography. Benzyltriethylammonium bromide(BTEAB) was used as a detection reagent. Also, the retention mechanism and response of samples were investigated to the several factors such as pH, temperature, and concentration of MeOH as well as concentration of detection reagents in mobile phase. And some mixture of samples were able to be separated under optimum condition.
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